Longevity Wiki - User contributions [en-GB]

Transposons in aging

2022-11-12T15:08:23Z

Wurstuk: removed comments

[[File:Transposition mechanism.png|thumb|[Write caption later]]]
Transposons were first discovered in corn (maize) by Barbara McClintock during the decades of 1940-50s, for which she won the Nobel Prize of Physiology or Medicine in 1983.<ref>McClintock, B. (1931). The Order of the Genes C, Sh and Wx in Zea Mays with Reference to a Cytologically Known Point in the Chromosome. ''Proceedings Of The National Academy Of Sciences'', ''17''(8), 485-491. doi: 10.1073/pnas.17.8.485</ref><ref>McClintock, B. Mutable loci in maize. Carnegie Institution of Washington Yearbook '''50''', 174–181 (1951).[https://profiles.nlm.nih.gov/spotlight/ll]</ref><ref>Ravindran, S. (2012). Barbara McClintock and the discovery of jumping genes. ''Proceedings Of The National Academy Of Sciences'', ''109''(50), [tel:20198-20199 20198-20199]. doi: 10.1073/pnas.1219372109</ref>

Transposons, also called "jumping genes", are genes not fixed in location and are capable of changing position within the genome. Transposase enzymes bind to the end of transposon elements and catalyze their movement to other parts of the genome. Transposons have no known direct functions, but they can sometimes result in ''de novo'' mutations or changes in genome size. This can result in dangerous modifications to the genome, as they might lead to inactivation of important sequences, aberrant control of genes or tumourogenesis.

Retrotransposons, the most common type of transposons, proceeds via an RNA intermediate that can be targeted by RNA interference (RNAi) mechanisms, which then degrade these molecules to prevent their expression. Thus, RNAi is postulated to have evolved as a protective mechanism against retroviruses and endogenous retrotransposons in order to prevent their excessive activity.

==Types of transposons==
There are several types of transposons:

===DNA-only transposons===
This type of transposons, and unlike retrotransposons, are able to translocate from one chromosome to another. They code themselves for transposase enzymes, which facilitates the transferring process. DNA-only transposons do not transfer very frequently and it is unknown what process is responsible for their action.
===Retrotransposons===
These are the most common class of transposons in humans. They are sections of DNA inserted into the chromosome, but never jump out of it to other chromosomes. Retrotansposons transcribe their sequence of DNA into RNA copies and require the enzyme reverse transcriptase to then be converted into double-stranded DNA, which will then insert themselves elsewhere in the genome. They are named after retroviruses such as HIV, which also require reverse transcriptase to be inserted into the host chromosomes and replicate. Retrotransposons have had an important role in evolution by generating a large amount of the repetitive DNA sequences, and much of the non-coding "junk" DNA consists of this type of transposons.
==Transposons in aging==

=== Transposons as a cause of aging===
Transposons are hypothesized to be a cause of aging due to some of their properties.<ref name=":0">Murray, V. (1990). Are transposons a cause of ageing?. ''Mutation Research/Dnaging'', ''237''(2), 59-63. doi: 10.1016/0921-8734(90)90011-f</ref> This hypothesis is based on the observation that duplication of genes during transposition, the process in which a transposon element moves to another location in the genome, might lead to inactivation of essential genes or to cause aberrant function. Thus dysfunctional processes during aging have been proposed to arise as a consequence of the large amount of transposon elements present in "junk" DNA regions, and might potentially explain the properties of senescent cells.<ref name=":0" />

===Transposons in laminopathic diseases===
Laminopathies are a type of rare genetic disorders that arise from mutations in nuclear lamina genes, which are important to maintain fibrillar networks in the nucleus and to regulate events in chromatin organisation. A reduction in the expression of some lamin genes is also associated with aging and to a deregulation of transposable elements.<ref>Andrenacci, D., Cavaliere, V., & Lattanzi, G. (2020). The role of transposable elements activity in aging and their possible involvement in laminopathic diseases. ''Ageing Research Reviews'', ''57'', 100995. doi: 10.1016/j.arr.2019.100995</ref> (*to expand)

<references />
[[Category:Longevity]]

Transposons in aging

2022-10-27T15:56:43Z

Wurstuk: Added an illustration about transposition mechanisms

[[File:Transposition mechanism.png|thumb|[Write caption later]]]
Transposons were first discovered in corn (maize) by Barbara McClintock during the decades of 1940-50s, for which she won the Nobel Prize of Physiology or Medicine in 1983.<ref>McClintock, B. (1931). The Order of the Genes C, Sh and Wx in Zea Mays with Reference to a Cytologically Known Point in the Chromosome. ''Proceedings Of The National Academy Of Sciences'', ''17''(8), 485-491. doi: 10.1073/pnas.17.8.485</ref><ref>McClintock, B. Mutable loci in maize. Carnegie Institution of Washington Yearbook '''50''', 174–181 (1951).[https://profiles.nlm.nih.gov/spotlight/ll]</ref><ref>Ravindran, S. (2012). Barbara McClintock and the discovery of jumping genes. ''Proceedings Of The National Academy Of Sciences'', ''109''(50), 20198-20199. doi: 10.1073/pnas.1219372109</ref>

Transposons, also called "jumping genes", are genes not fixed in location and are capable of changing position within the genome. Transposase enzymes bind to the end of transposon elements and catalyze their movement to other parts of the genome. Transposons have no known direct functions, but they can sometimes result in ''de novo'' mutations or changes in genome size. This can result in dangerous modifications to the genome, as they might lead to inactivation of important sequences, aberrant control of genes or tumourogenesis.

Retrotransposons, the most common type of transposons, proceeds via an RNA intermediate that can be targeted by RNA interference (RNAi) mechanisms, which then degrade these molecules to prevent their expression. Thus, RNAi is postulated to have evolved as a protective mechanism against retroviruses and endogenous retrotransposons in order to prevent their excessive activity.

==Types of transposons==
There are several types of transposons:

===DNA-only transposons===
This type of transposons, and unlike retrotransposons, are able to translocate from one chromosome to another. They code themselves for transposase enzymes, which facilitates the transferring process. DNA-only transposons do not transfer very frequently and it is unknown what process is responsible for their action.

<code>Anton:</code> ''As far as I know the major difference between DNA (without only) transposons and retrotransposons is, that DNA transposons transpose via a "cut-and-paste" mechanism and retrotransposons via a "copy-and-paste" mechanism. I think there are numerous great illustrations of this and I can look them up later :)''

===Retrotransposons===
These are the most common class of transposons in humans. They are sections of DNA inserted into the chromosome, but never jump out of it to other chromosomes. Retrotansposons transcribe their sequence of DNA into RNA copies and require the enzyme reverse transcriptase to then be converted into double-stranded DNA, which will then insert themselves elsewhere in the genome. They are named after retroviruses such as HIV, which also require reverse transcriptase to be inserted into the host chromosomes and replicate. Retrotransposons have had an important role in evolution by generating a large amount of the repetitive DNA sequences, and much of the non-coding "junk" DNA consists of this type of transposons.

<code>Anton:</code> ''I think [[wikipedia:Long_interspersed_nuclear_element|Lines]] are also quite important transposons for humans (esp. LINE1) but I don't know if they are important for aging.''

==Transposons in aging==

=== Transposons as a cause of aging===
Transposons are hypothesized to be a cause of aging due to some of their properties.<ref name=":0">Murray, V. (1990). Are transposons a cause of ageing?. ''Mutation Research/Dnaging'', ''237''(2), 59-63. doi: 10.1016/0921-8734(90)90011-f</ref> This hypothesis is based on the observation that duplication of genes during transposition, the process in which a transposon element moves to another location in the genome, might lead to inactivation of essential genes or to cause aberrant function. Thus dysfunctional processes during aging have been proposed to arise as a consequence of the large amount of transposon elements present in "junk" DNA regions, and might potentially explain the properties of senescent cells.<ref name=":0" />

===Transposons in laminopathic diseases===
Laminopathies are a type of rare genetic disorders that arise from mutations in nuclear lamina genes, which are important to maintain fibrillar networks in the nucleus and to regulate events in chromatin organisation. A reduction in the expression of some lamin genes is also associated with aging and to a deregulation of transposable elements.<ref>Andrenacci, D., Cavaliere, V., & Lattanzi, G. (2020). The role of transposable elements activity in aging and their possible involvement in laminopathic diseases. ''Ageing Research Reviews'', ''57'', 100995. doi: 10.1016/j.arr.2019.100995</ref> (*to expand)

<references />

File:Transposition mechanism.png

2022-10-27T15:53:08Z

Wurstuk:

Overview about major types of transposition mechanisms.

Transposons in aging

2022-10-26T16:42:50Z

Wurstuk: only commented

Transposons were first discovered in corn (maize) by Barbara McClintock during the decades of 1940-50s, for which she won the Nobel Prize of Physiology or Medicine in 1983.<ref>McClintock, B. (1931). The Order of the Genes C, Sh and Wx in Zea Mays with Reference to a Cytologically Known Point in the Chromosome. ''Proceedings Of The National Academy Of Sciences'', ''17''(8), 485-491. doi: 10.1073/pnas.17.8.485</ref><ref>McClintock, B. Mutable loci in maize. Carnegie Institution of Washington Yearbook '''50''', 174–181 (1951).[https://profiles.nlm.nih.gov/spotlight/ll]</ref><ref>Ravindran, S. (2012). Barbara McClintock and the discovery of jumping genes. ''Proceedings Of The National Academy Of Sciences'', ''109''(50), 20198-20199. doi: 10.1073/pnas.1219372109</ref>

Transposons, also called "jumping genes", are genes not fixed in location and are capable of changing position within the genome. Transposase enzymes bind to the end of transposon elements and catalyze their movement to other parts of the genome. Transposons have no known direct functions, but they can sometimes result in ''de novo'' mutations or changes in genome size. This can result in dangerous modifications to the genome, as they might lead to inactivation of important sequences, aberrant control of genes or tumourogenesis.

Retrotransposons, the most common type of transposons, proceeds via an RNA intermediate that can be targeted by RNA interference (RNAi) mechanisms, which then degrade these molecules to prevent their expression. Thus, RNAi is postulated to have evolved as a protective mechanism against retroviruses and endogenous retrotransposons in order to prevent their excessive activity.

== Types of transposons ==
There are several types of transposons:

=== DNA-only transposons ===
This type of transposons, and unlike retrotransposons, are able to translocate from one chromosome to another. They code themselves for transposase enzymes, which facilitates the transferring process. DNA-only transposons do not transfer very frequently and it is unknown what process is responsible for their action.

<code>Anton:</code> ''As far as I know the major difference between DNA (without only) transposons and retrotransposons is, that DNA transposons transpose via a "cut-and-paste" mechanism and retrotransposons via a "copy-and-paste" mechanism. I think there are numerous great illustrations of this and I can look them up later :)''

=== Retrotransposons ===
These are the most common class of transposons in humans. They are sections of DNA inserted into the chromosome, but never jump out of it to other chromosomes. Retrotansposons transcribe their sequence of DNA into RNA copies and require the enzyme reverse transcriptase to then be converted into double-stranded DNA, which will then insert themselves elsewhere in the genome. They are named after retroviruses such as HIV, which also require reverse transcriptase to be inserted into the host chromosomes and replicate. Retrotransposons have had an important role in evolution by generating a large amount of the repetitive DNA sequences, and much of the non-coding "junk" DNA consists of this type of transposons.

<code>Anton:</code> ''I think [[wikipedia:Long_interspersed_nuclear_element|Lines]] are also quite important transposons for humans (esp. LINE1) but I don't know if they are important for aging.''

== Transposons in aging ==

=== Transposons as a cause of aging ===
Transposons are hypothesized to be a cause of aging due to some of their properties.<ref name=":0">Murray, V. (1990). Are transposons a cause of ageing?. ''Mutation Research/Dnaging'', ''237''(2), 59-63. doi: 10.1016/0921-8734(90)90011-f</ref> This hypothesis is based on the observation that duplication of genes during transposition, the process in which a transposon element moves to another location in the genome, might lead to inactivation of essential genes or to cause aberrant function. Thus dysfunctional processes during aging have been proposed to arise as a consequence of the large amount of transposon elements present in "junk" DNA regions, and might potentially explain the properties of senescent cells.<ref name=":0" />

=== Transposons in laminopathic diseases ===
Laminopathies are a type of rare genetic disorders that arise from mutations in nuclear lamina genes, which are important to maintain fibrillar networks in the nucleus and to regulate events in chromatin organisation. A reduction in the expression of some lamin genes is also associated with aging and to a deregulation of transposable elements.<ref>Andrenacci, D., Cavaliere, V., & Lattanzi, G. (2020). The role of transposable elements activity in aging and their possible involvement in laminopathic diseases. ''Ageing Research Reviews'', ''57'', 100995. doi: 10.1016/j.arr.2019.100995</ref> (*to expand)

Killifish as an aging animal model

2022-09-17T18:06:59Z

Wurstuk: Added short paragraph to lifespan about lifespan extension.

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Aging_features Aging features]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

[[Calorie restriction|Dietary restriction]] can increase the maximum lifespan for both the short-lived GRZ laboratory strain and the longer-lived wild-derived strain MZM-04/10P.<ref name=":10" /> However, in the wild strain MZM-04/10P, lifespan extension is associated with increased baseline mortality.<ref name=":10" /> In addition to dietary restriction, lowering the water temperature also increased the median lifespan significantly.<ref name=":9" />
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
[[Telomeres]] are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short [[telomeres]].<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the [[telomeres]] of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering water temperature (for instance from 25°C to 22°C) as well as [[Calorie restriction|dietary restriction]] can reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
[[Mitochondria]] are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike in mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has decreased levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) might be impaired in old killifish (see also [[Mitochondrial dysfunction]]).<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called [[Cellular senescence|replicative senescence]]. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by markers such as the appearance of senescence-associated β-galactosidase (β-GAL).<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased β-GAL activity,<ref name=":8" /><ref name=":12" /> which is attenuated with lifespan-extending interventions like lowering water temperature.<ref name=":9" />

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his contribution in characterising the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

The turquoise killifish has been recently established as a model for [[Aging and eye disease|age-related eye disease]].<ref name=":14">Vanhunsel, S., Bergmans, S., Beckers, A., Etienne, I., Van houcke, J., & Seuntjens, E. et al. (2021). The killifish visual system as an in vivo model to study brain aging and rejuvenation. ''Npj Aging And Mechanisms Of Disease'', ''7''(1). doi: 10.1038/s41514-021-00077-4</ref> Considering vision decline as a conserved aging hallmark, the aging-associated decline of the killifish visual system has been proposed as a useful ''in vivo'' model to study brain aging and rejuvenation.<ref name=":14" />

====Genetic tools available====
The group led by Anne Brunet in Stanford University has developed a genotype-to-phenotype platform using de-novo-assembled genome and CRISPR/Cas9 technology, which allows for high-throughput and high efficiency knock-out and knock-in studies in killifish.<ref name=":15">Harel, I., Benayoun, B., Machado, B., Singh, P., Hu, C., & Pech, M. et al. (2015). A Platform for Rapid Exploration of Aging and Diseases in a Naturally Short-Lived Vertebrate. ''Cell'', ''160''(5), 1013-1026. doi: 10.1016/j.cell.2015.01.038</ref>

A key tool for generating transgenic species in fish is the transposase system. Transposon elements (TE) are genes capable of changing position within the genome, which can sometimes result in ''de novo'' mutations or changes in genome size.<ref>Bourque, G., Burns, K., Gehring, M., Gorbunova, V., Seluanov, A., & Hammell, M. et al. (2018). Ten things you should know about transposable elements. ''Genome Biology'', ''19''(1). doi: 10.1186/s13059-018-1577-z</ref> Transposase enzymes bind to the end of TE and catalyze their movement to other parts of the genome. In killifish, the Tol2 transposase system has been adapted from other model animals by Valenzano to integrate genes of interest into the host's genome in a stable and efficient manner.<ref>Valenzano, D., Sharp, S., & Brunet, A. (2011). Transposon-Mediated Transgenesis in the Short-Lived African KillifishNothobranchius furzeri, a Vertebrate Model for Aging. ''G3 Genes|Genomes|Genetics'', ''1''(7), 531-538. doi: 10.1534/g3.111.001271</ref>

Additional progress has been made in developing other genetic tools for killifish. <ref>Harel, I., Valenzano, D., & Brunet, A. (2016). Efficient genome engineering approaches for the short-lived African turquoise killifish. ''Nature Protocols'', ''11''(10), 2010-2028. doi: 10.1038/nprot.2016.103</ref><ref>Platzer, M., & Englert, C. (2016). Nothobranchius furzeri: A Model for Aging Research and More. ''Trends In Genetics'', ''32''(9), 543-552. doi: 10.1016/j.tig.2016.06.006</ref> Due to the fast life cycle of killifish, new stable transgenic lines can be generated as rapidly as in 2 to 3 months.<ref name=":15" />

==Limitations of killifish==
Whilst killifish can be a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it may even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-12T06:39:36Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Aging_features Aging features]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
[[Telomeres]] are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short [[telomeres]].<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the [[telomeres]] of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
[[Mitochondria]] are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish (see also [[Mitochondrial dysfunction]]).<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called [[Cellular senescence|replicative senescence]]. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-12T06:38:11Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Aging_features Aging features]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
[[Telomeres]] are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short [[telomeres]].<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the [[telomeres]] of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
[[Mitochondria]] are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish (see also [[Mitochondrial dysfunction]]).<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-12T06:35:48Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Aging_features Aging features]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
[[Telomeres]] are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short [[telomeres]].<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the [[telomeres]] of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T20:39:31Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Aging_features Aging features]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:26:35Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:24:48Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|319x319px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:23:16Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|227x227px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:22:14Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

[[File:Turquoise killifish habitat.png|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]

==Habitat and life cycle of the turquoise killifish==
[[File:Killifish life cycle schematic.png|thumb|250x250px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:20:09Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

[[File:Turquoise killifish habitat.png|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
[[File:Killifish life cycle schematic.png|thumb|250x250px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]

==Habitat and life cycle of the turquoise killifish==
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:19:04Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

[[File:Turquoise killifish habitat.png|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
==Habitat and life cycle of the turquoise killifish==
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
[[File:Killifish life cycle schematic.png|thumb|250x250px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T17:17:31Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
[[File:Killifish life cycle schematic.png|thumb|250x250px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
==Aging features==

===Macroscopic changes===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

===Regenerative capacity===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
===Mobility===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

===Cognitive decline===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Molecular markers of Ageing==

===Telomere length===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

===Lipofuscin===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

===Mitochondria===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

===Replicative senescence===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

*Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

==References==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T16:05:30Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
=== Mobility ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

=== Mitochondria ===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

=== Replicative senescence ===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T16:04:48Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>|231x231px]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|239x239px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>

The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. During the rainy season, the killifishes hatch and rapidly develop to reach sexual maturity. Subsequently, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
=== Mobility ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

=== Mitochondria ===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

=== Replicative senescence ===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T15:29:10Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
=== Mobility ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduce age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

=== Mitochondria ===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

=== Replicative senescence ===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T15:12:22Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
=== Mobility ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but upregulation can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduced age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

=== Mitochondria ===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

=== Replicative senescence ===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T15:11:26Z

Wurstuk: Added content to Section about molecular markers

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>
=== Mobility ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C (instead of 25 °C) significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />

Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but it can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref name=":12">Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduced age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

=== Mitochondria ===
Mitochondria are the primary energy providers of most eukaryotic cells, and unlike other organelles, they are unique in that they contain their own DNA (mtDNA). Although large-scale, age-dependent mtDNA deletions are not observed in old killifish (unlike for mammals), the DNA copy number in old killifish decreases with age.<ref name=":13">Hartmann, N., Reichwald, K., Wittig, I., Dröse, S., Schmeisser, S., Lück, C., Hahn, C., Graf, M., Gausmann, U., Terzibasi, E., Cellerino, A., Ristow, M., Brandt, U., Platzer, M., & Englert, C. (2011). Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri. ''Aging Cell'', ''10''(5), 824–831. https://doi.org/10.1111/j.1474-9726.2011.00723.x</ref> Overall, old killifish show lower expression of mitochindria-associated proteins such as Pgc-1a, Tfam, and mtSsbp, and old muscle tissue has lower levels of respiratory chain complexes.<ref name=":13" /> This indicates that oxidative phosphorylation (the process of energy production in mitochondria) is impaired in old killifish.<ref name=":13" />

=== Replicative senescence ===
After a certain number of replications, cells enter a state of growth arrest and altered function called replicative senescence. Replicative senescence is considered a [[Hallmarks of aging|hallmark of aging]], and senescent cells can be detected by the appearance of senescence-associated β-galactosidase.<ref>Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995). A biomarker that identifies senescent human cells in culture and in aging skin in vivo. ''Proceedings of the National Academy of Sciences of the United States of America'', ''92''(20), 9363–9367. https://doi.org/10.1073%2Fpnas.92.20.9363</ref> Old killifish show increased senescence-associated β-galactosidase activity<ref name=":8" /><ref name=":12" />, which can be attenuated by lowering the water temperature<ref name=":9" />.

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-11T13:09:13Z

Wurstuk: Created the section "Molecular markers of Ageing" and added subsection to telomeres and lipofuscin

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

=== Open-field exploration ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref>

=== Mobility ===
Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C instead of 25 °C significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

== Molecular markers of Ageing ==

=== Telomere length ===
Telomeres are protective caps at the ends of chromosomes that become shorter with age in various organisms. The telomere length of the short-lived killifish strain GRZ does not shorten with age, suggesting that the shorter lifespan of the strain is not a result of short telomeres.<ref name=":11">Hartmann, N., Reichwald, K., Lechel, A., Graf, M., Kirschner, J., Dorn, A., Terzibasi, E., Wellner, J., Platzer, M., Rudolph, K. L., Cellerino, A., & Englert, C. (2009). Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri. ''Mechanisms of Ageing and Development'', ''130''(5), 290–296. https://doi.org/10.1016/j.mad.2009.01.003</ref> On the other hand, the telomeres of the longer-lived strain MZM0403 show significant telomere shortening within 16 weeks of life.<ref name=":11" /> Surprisingly, no upregulation of the telomere-preserving enzyme telomerase can be observed in old individuals of the short-lived strain GRZ, but it can be observed in the longer-lived strain MZM0403.<ref name=":11" />

=== Lipofuscin ===
Lipofuscin, or age pigment, is an autofluorescent pigment that accumulates progressively with age within the cells of many species.<ref>Brunk, U. T., Jones, C. B., & Sohal, R. S. (1992). A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. ''Mutation Research'', ''275''(3–6), 395–403. https://doi.org/10.1016/0921-8734(92)90042-n</ref> In old killifish, elevated lipofuscin levels are detected in various cell types such as heart and liver cells.<ref name=":7" /><ref name=":8" /><ref>Ahuja et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. ''EMBO Reports'', ''20''(4), e47407. https://doi.org/10.15252/embr.201847407</ref> Thereby, lipofuscin accumulation is faster in the short-lived strain GRZ than in the longer-lived strain MZM0403, suggesting that the short lifespan of the GRZ strain is associated with faster histological aging.<ref name=":7" />

Lowering the water temperature from 25 °C to 22 °C and [[Calorie restriction|dietary restriction]] reduced age-related lipofuscin accumulation in old killifish.<ref name=":9" /><ref name=":10" />

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-09-05T18:32:25Z

Wurstuk:

{{Draft-article}}[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

=== Open-field exploration ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref>

=== Mobility ===
Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22 °C instead of 25 °C significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25 °C to 22 °C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Animal model for aging research==
[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-20T13:08:54Z

Wurstuk: changed headline format and added paragraph to ageing phenotype

[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]{{Draft-article}}
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

== Habitat and life cycle of the turquoise killifish ==
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

==Lifespan==
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
== Aging features ==

=== Macroscopic changes ===
[[File:Young and old killifish.png|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref>

Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" />

=== Regenerative capacity ===
Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

=== Open-field exploration ===
Open-field exploration is a standard behavioural test used in rodents that quantifies the amount of time an individual explores a new environment. Old killifish spend significantly less time exploring new environments compared to young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref>

=== Mobility ===
Killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> However, interventions with [[resveratrol]] or reducing the water temperature to 22°C instead of 25°C significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

=== Cognitive decline ===
To evaluate cognitive decline in aged killifish the active avoidance test is used. In this test, fish make an association between a red light and punishment. Both young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" />
[[File:Age-dependent neurodegeneration in Killifish.png|thumb|Neurodegeneration in Stratum Griseum Superficiale of the Optic Tectum of Killifish and the effect of resveratrol detected by Fluoro-Jade B staining.<ref name=":6" />]]
Remarkably, the age-related decline in cognitive performance is completely prevented in old killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and reducing temperature from 25ºC to 22°C also attenuates age-related cognitive decline.<ref name=":10">Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

Additionally, old Killifish show molecular since of neurodegeneration, as detected by Fluoro-Jade B staining.<ref name=":6" /><ref name=":7" /> and neurodegeneration is accelerated in short-lived strains compared with longer-lived ones.<ref name=":7" /> [[Calorie restriction|Dietary restriction]] and [[resveratrol]] can reduce age-dependent neurodegeneration.<ref name=":6" /><ref name=":10" /> Furthermore, old Killifish can show pathological phenotypes similar to Parkinson’s disease.<ref>Matsui, Hideaki, Naoya Kenmochi, and Kazuhiko Namikawa. 2019. ‘Age- and α-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius Furzeri’. ''Cell Reports'' 26(7):1727-1733.e6. https://doi.org/10.1016/j.celrep.2019.01.015</ref>

==Animal model for aging research==

Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.

One of the main advantages of using killifish as an aging animal model is its close resemblance to human aging, usually seen in much longer lived animal models such as mice and zebrafish, and its short lifespan of 4 to 8 months, which allows for greater experimental scalability.<ref name=":02">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref>

====Genetic tools available====
https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

====Killifish as a model to study brain aging====
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/

==Limitations of killifish==
Whilst killifish are a great alternative compared to more conventional animal models, they also suppose some limitations:

* Teleost fish such as killifish, which includes a large and very diverse group of ray-finned fishes, all possess a duplicated genome. This whole-genome duplication (WGD) occurred in an ancient common ancestor of all teleost fishes. Duplicated genes may sometimes serve different functions or lead to the non-functionalization of one of the genes.<ref>Glasauer, S., & Neuhauss, S. (2014). Whole-genome duplication in teleost fishes and its evolutionary consequences. ''Molecular Genetics And Genomics'', ''289''(6), 1045-1060. doi: 10.1007/s00438-014-0889-2</ref> This WGD might have occurred at least twice during evolution and may have led to highly fish-specific adaptations.<ref>Dehal, P., & Boore, J. (2005). Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate. ''Plos Biology'', ''3''(10), e314. doi: 10.1371/journal.pbio.0030314</ref> This poses significant limitations on findings derived from killifishes.
*Each killifish requires approximately 1 liter of water.<ref>Dodzian, J., Kean, S., Seidel, J., & Valenzano, D. (2018). A Protocol for Laboratory Housing of Turquoise Killifish (&lt;em&gt;Nothobranchius furzeri&lt;/em&gt;). ''Journal Of Visualized Experiments'', (134). doi: 10.3791/57073</ref> This aspect becomes very troublesome for high throughput assays when taking into account space logistics. Furthermore, it can even prohibit pharmacological approaches, given that incredibly large amounts of drugs would need to be diluted in 1L of water to potentially have an effect.

== References ==
<references />
[[Category:Longevity]]
[[Category:Drafts]]

File:Age-dependent neurodegeneration in Killifish.png

2022-08-20T12:39:32Z

Wurstuk:

Age-dependent neurodegeneration in Killifish

Killifish as an aging animal model

2022-08-14T16:32:49Z

Wurstuk:

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
=== Aging phenotype ===
[[File:Young and old killifish.png|left|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]
Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref> Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" /> Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

Open-field exploration is a standard behavioral test in rodents that quantifies exploring a new environment. Old Killifish spend significantly less time exploring new environments compared with young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Moreover, killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> Setting the water temperature to 22 °C instead of 25°C and administration of [[resveratrol]] significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

To evaluate cognitive decline in aged Killifish the active avoidance test is used. In this test, the fish has to make an association between the illumination of a red light and a punishment. Young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" /> The age-related decline in cognitive performance was completely prevented in old Killifish treated with [[resveratrol]].<ref name=":6" /> In addition, [[Calorie restriction|dietary restriction]] and a temperature reduction to 22 °C also attenuated the age-related cognitive decline.<ref>Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-14T16:26:40Z

Wurstuk:

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
=== Aging phenotype ===
[[File:Young and old killifish.png|left|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]
Like most animals, killifishes show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref> Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" /> Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

Open-field exploration is a standard behavioral test in rodents that quantifies exploring a new environment. Old Killifish spend significantly less time exploring new environments compared with young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Moreover, killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> Setting the water temperature to 22 °C instead of 25°C and administration of resveratrol significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

To evaluate cognitive decline in aged Killifish the active avoidance test is used. In this test, the fish has to make an association between the illumination of a red light and a punishment. Young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" /> The age-related decline in cognitive performance was completely prevented in old Killifish treated with resveratrol.<ref name=":6" /> In addition, dietary restriction and a temperature reduction to 22 °C also attenuated the age-related cognitive decline.<ref>Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-14T16:18:24Z

Wurstuk: added paragraphs to Aging phenotype (WIP)

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref name=":6">Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
=== Aging phenotype ===
[[File:Young and old killifish.png|left|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]
Like most animals, killifishes also show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref name=":7">Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /><ref name=":8">Genade, Tyrone, Mauro Benedetti, Eva Terzibasi, Paola Roncaglia, Dario Riccardo Valenzano, Antonino Cattaneo, and Alessandro Cellerino. 2005. ‘Annual Fishes of the Genus Nothobranchius as a Model System for Aging Research’. ''Aging Cell'' 4(5):223–33. https://doi.org/10.1111/j.1474-9726.2005.00165.x</ref> Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, while fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype.<ref name=":7" /> Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, whereas young fish can regenerate them almost completely.<ref>Wendler, Sebastian, Nils Hartmann, Beate Hoppe, and Christoph Englert. 2015. ‘Age-Dependent Decline in Fin Regenerative Capacity in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 14(5):857–66. https://doi.org/10.1111/acel.12367</ref>

Open-field exploration is a standard behavioral test in rodents that quantifies exploring a new environment. Old Killifish spend significantly less time exploring new environments compared with young fish and show a decreased moving velocity.<ref name=":6" /><ref name=":7" /><ref name=":8" /><ref name=":9">Valenzano, Dario R., Eva Terzibasi, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Temperature Affects Longevity and Age-Related Locomotor and Cognitive Decay in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 5(3):275–78. https://doi.org/10.1111/j.1474-9726.2006.00212.x</ref> Moreover, killifishes generally show less spontaneous movement and swimming as they age.<ref name=":6" /><ref name=":8" /> Setting the water temperature to 22 °C instead of 25°C and administration of resveratrol significantly reduces age-related mobility deficits.<ref name=":6" /><ref name=":9" />

To evaluate cognitive decline in aged Killifish the active avoidance test is used. In this test, the fish has to make an association between the illumination of a red light and a punishment. Young and old fish succeed in learning the task, but young fish show significantly higher success rates than old fish.<ref name=":6" /><ref name=":7" /><ref name=":9" /> The age-related decline in cognitive performance was completely prevented in old Killifish treated with resveratrol.<ref name=":6" /> In addition, dietary restriction and a temperature reduction to 22 °C also attenuated the age-related cognitive decline.<ref>Terzibasi, Eva, Christel Lefrançois, Paolo Domenici, Nils Hartmann, Michael Graf, and Alessandro Cellerino. 2009. ‘Effects of Dietary Restriction on Mortality and Age-Related Phenotypes in the Short-Lived Fish Nothobranchius Furzeri’. ''Aging Cell'' 8(2):88–99. https://doi.org/10.1111/j.1474-9726.2009.00455.x</ref><ref name=":9" />

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-14T15:07:06Z

Wurstuk: Added part to Aging phenotype (WIP)

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref>Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
=== Aging phenotype ===
[[File:Young and old killifish.png|left|thumb|With age, the fish lose their body color, the fin structure deteriorates, and the spine becomes curved. <ref name=":5">Kim, Yumi, Hong Gil Nam, and Dario Riccardo Valenzano. 2016. ‘The Short-Lived African Turquoise Killifish: An Emerging Experimental Model for Ageing’. ''Disease Models & Mechanisms'' 9(2):115–29. https://doi.org/10.1242/dmm.023226</ref>]]
Like most animals, killifishes also show macroscopic signs of aging like a loss of color and pigmentation, emaciation, and a curved spine.<ref name=":5" /><ref>Terzibasi, Eva, Dario Riccardo Valenzano, Mauro Benedetti, Paola Roncaglia, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2008. ‘Large Differences in Aging Phenotype between Strains of the Short-Lived Annual Fish Nothobranchius Furzeri’. ''PLOS ONE'' 3(12):e3866. https://doi.org/10.1371/journal.pone.0003866</ref> The loss of color is more pronounced in males as they are more colorful than females, whereas females tend to lose their rotund appearance due to a prominent curved spine.<ref name=":5" /> (Genade et al. 2005; Kim et al. 2016) Killifishes show substantial strain-dependent variation in the duration of this decrepit state. Fish from wild strains can remain in this state for several weeks before they finally die, whereas fish of the short-lived GRZ strain usually die before developing a macroscopic phenotype. (Terzibasi et al. 2008) Besides an overall decrepit appearance, killifishes also show an impaired ability to regenerate the caudal fins with age, while young fish can regenerate them almost completely. (Wendler et al. 2015)

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

File:Young and old killifish.png

2022-08-14T13:54:37Z

Wurstuk:

Difference in young and old killifish

Killifish as an aging animal model

2022-08-14T13:19:04Z

Wurstuk: added links for further research on lifespan section.

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref>Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/
*https://doi.org/10.1242/dmm.023226
[[File:Survival curve for turquoise killifish.jpg|left|thumb|Typical survival curve for laboratory-raised GRZ turquoise killifish.<ref name=":3" /> MAYBE NOT IMPORTANT]]

=== Aging phenotype ===
https://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2005.00165.x

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-13T18:26:15Z

Wurstuk: added links for further research on lifespan section.

[[File:Nothobranchius furzeri GRZ thumb.jpg|thumb|Male ''Nothobranchius furzeri'', also known as the turquoise killifish.<ref>https://en.wikipedia.org/wiki/Nothobranchius_furzeri</ref>]]
The African turquoise killifish (''Nothobranchius furzeri'') is the shortest lived vertebrate known to date, with a median lifespan of only 4 to 8 months. This short lifespan is closer to that of unicellular and invertebrate research organisms (such as the fruit fly) than to vertebrate research organisms such as mice or zebrafish.<ref name=":0">Hu, C., & Brunet, A. (2018). The African turquoise killifish: A research organism to study vertebrate aging and diapause. ''Aging Cell'', ''17''(3), e12757. [https://doi.org/10.1111/acel.12757 doi: 10.1111/acel.12757]</ref> This places the Turquoise Killifish in the unique position of allowing high repeatability and feasibility for experiments, whilst better recapitulating human aging traits.

=== Habitat and life cycle of the turquoise killifish ===
[[File:Turquoise killifish habitat.png|left|thumb|249x249px|The turquoise killifish inhabits ponds of fresh water, which entirely dry out during the dry season. (A) Turquoise killifish habitat one week after filling with water. (B) The same habitat fully desiccated. <ref>Reichard, M., & Polačik, M. (2019). Nothobranchius furzeri, an “instant” fish from an ephemeral habitat. ''eLife'', ''8''. https://doi.org/10.7554/elife.41548</ref>]]
The turquoise killifish's natural habitat is in freshwater ponds throughout central and eastern Africa, specifically the regions of Zimbabwe and Mozambique.<ref>''Nothobranchius furzeri summary page''. FishBase. Retrieved July 22, 2022, from https://www.fishbase.de/summary/Nothobranchius-furzeri.html</ref>

Due to alternating dry and rainy seasons, these regions have pronounced seasonal differences in water availability, causing the turquoise killifish to inhabit reservoirs of water that fill up during short rainy seasons and dry out entirely during the longer dry seasons.

To survive as a species, the turquoise killifish has developed a unique annual life cycle in which it can persist periods of drought through an extended period of embryonic stasis called diapause.<ref>Poeschla, M., & Valenzano, D. R. (2020). The turquoise killifish: A genetically tractable model for the study of aging. ''Journal of Experimental Biology'', ''223''(Suppl_1), jeb209296. https://doi.org/10.1242/jeb.209296</ref> With the onset of the wet season, the turquoise killifish then switches to a mode of explosive growth and sexual maturation, resulting in females laying up to 120 eggs per day.<ref>Vrtílek, M., & Reichard, M. (2016). Female fecundity traits in wild populations of African annual fish: the role of the aridity gradient. ''Ecology and Evolution'', ''6''(16), 5921–5931. https://doi.org/10.1002/ece3.2337</ref>
[[File:Killifish life cycle schematic.png|thumb|278x278px|The life cycle of killifish consists of two main stages: natural lifespan and diapause state. In January, the short inundated phase starts with the onset of the rainy season. During this phase, the killifishes hatch and rapidly develop to reach sexual maturity in about 14 days. During the remaining rainy season, the killifish mate and constantly produce eggs. The eggs remain in a diapause state to survive the following dry phase, where the whole pond is desiccated, and all killifish die. <ref name=":1">Valenzano, D. R., Benayoun, B. A., Singh, P. P., Zhang, E., Etter, P. D., Hu, C.-K., Clément-Ziza, M., Willemsen, D., Cui, R., Harel, I., Machado, B. E., Yee, M.-C., Sharp, S. C., Bustamante, C. D., Beyer, A., Johnson, E. A., & Brunet, A. (2015). The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. ''Cell'', ''163''(6), 1539–1554. <nowiki>https://doi.org/10.1016/j.cell.2015.11.008</nowiki></ref>]]
The process from hatching to sexually mature fish takes no more than 14 days, making it the fastest known rate of sexual maturation for vertebrates.<ref name=":2">Vrtílek, M., Žák, J., Pšenička, M., & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. ''Current Biology'', ''28''(15), R822–R824. https://doi.org/10.1016/j.cub.2018.06.031</ref> Subsequently, the fertilized eggs enter the diapause state to endure the following dry season, and the circle starts again. As a result of this annual life cycle, the turquoise killifish has a short adult lifespan of four to eight months and displays aging-related transformations like lose of body colour and specific patterning (''see section'' [https://en.longevitywiki.org/index.php?title=Killifish_as_an_Aging_Animal_Model#Animal_model_for_aging_research Animal model for aging research]).

===Lifespan===
While other standard model organisms such as mice and zebrafish have an average life expectancy of over 2.5 years in captivity, turquoise killifish live only four to eight months on average.<ref>Anon. 2017. ‘Non-Canonical Aging Model Systems and Why We Need Them’. ''The EMBO Journal'' 36(8):959–63. https://doi.org/10.15252/embj.201796837.</ref> Thereby, the lifespan of turquoise killifish is strongly strain-dependent. The original laboratory strain GRZ has the shortest lifespan of 11 to 18 weeks <ref name=":1" /><ref name=":3">Dodzian, Joanna, Sam Kean, Jens Seidel, and Dario Riccardo Valenzano. 2018. ‘A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)’. ''JoVE (Journal of Visualized Experiments)'' (134):e57073. https://doi.org/10.3791/57073</ref><ref name=":4">Kirschner, Jeanette, David Weber, Christina Neuschl, Andre Franke, Marco Böttger, Lea Zielke, Eileen Powalsky, Marco Groth, Dmitry Shagin, Andreas Petzold, Nils Hartmann, Christoph Englert, Gudrun A. Brockmann, Matthias Platzer, Alessandro Cellerino, and Kathrin Reichwald. 2012. ‘Mapping of Quantitative Trait Loci Controlling Lifespan in the Short-Lived Fish Nothobranchius Furzeri– a New Vertebrate Model for Age Research’. ''Aging Cell'' 11(2):252–61. https://doi.org/10.1111/j.1474-9726.2011.00780.x</ref>, while longer-lived strains like MZM0403 have a lifespan of 30 weeks<ref name=":4" />. Besides the strain, the lifespan also depends on diet, feeding frequency, and housing conditions.<ref name=":3" />

Although no sex-dependent difference in life expectancy is found in captivity<ref name=":1" /><ref name=":4" /><ref>Valenzano, Dario R., Eva Terzibasi, Tyrone Genade, Antonino Cattaneo, Luciano Domenici, and Alessandro Cellerino. 2006. ‘Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate’. ''Current Biology'' 16(3):296–300. https://doi.org/10.1016/j.cub.2005.12.038</ref>, significant differences in sex ratios are observed in the wild<ref>Reichard, M., M. Polačik, and O. Sedláček. 2009. ‘Distribution, Colour Polymorphism and Habitat Use of the African Killifish Nothobranchius Furzeri, the Vertebrate with the Shortest Life Span’. ''Journal of Fish Biology'' 74(1):198–212. https://doi.org/10.1111/j.1095-8649.2008.02129.x</ref>. During the rainy season, the proportion of the male population decreases, so that after three months at least two thirds of the population are female.<ref>Vrtílek, Milan, Jakub Žák, Matej Polačik, Radim Blažek, and Martin Reichard. 2018. ‘Longitudinal Demographic Study of Wild Populations of African Annual Killifish’. ''Scientific Reports'' 8(1):4774. https://doi.org/10.1038/s41598-018-22878-6</ref>

'''Add part about life span extending!'''

* https://www.cell.com/current-biology/abstract/S0960-9822(06)01020-7
* https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00455.x
* https://pubmed.ncbi.nlm.nih.gov/16842500/

[[File:Survival curve for turquoise killifish.jpg|left|thumb|Typical survival curve for laboratory-raised GRZ turquoise killifish.<ref name=":3" /> MAYBE NOT IMPORTANT]]

=== Aging phenotype ===
https://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2005.00165.x

===Animal model for aging research===
Dr Dario Valenzano, trained at Brunet lab in Stanford and currently at the Leibniz Institute on Aging, is especially known for his characterisation of the turquoise killifish. He is considered to have established this species as a novel research model and as an aging animal model.[[File:The African turquoise killifish as a novel animal model to study aging.jpg|thumb|The African turquoise killifish as a novel animal model to study aging. Being a vertebrate with a short lifespan of 4-6 months places the killifish in an unique position to study aging.<ref name=":0" />]]

* The unique features of the killifish as a fast vertebrate aging animal model

* Housing and caring protocols
** https://www.jove.com/de/t/57073/a-protocol-for-laboratory-housing-turquoise-killifish-nothobranchius
* Genetic tools available
* <nowiki>https://onlinelibrary.wiley.com/doi/10.1111/acel.12757</nowiki>

===Biological features conserved in killifish===
Figure 2 on: https://onlinelibrary.wiley.com/doi/10.1111/acel.12757

===Killifish as a model to study brain aging===
The killifish visual system as an in vivo model to study brain aging and rejuvenation:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371010/
<references />
[[Category:Longevity]]
[[Category:Drafts]]

Killifish as an aging animal model

2022-08-07T20:31:58Z

Wurstuk:

Killifish as an aging animal model

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Wurstuk:

Killifish life cycle schematic

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Survival curve for turquoise killifish

Killifish as an aging animal model

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Killifish as an aging animal model

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File:Killifish life cycle.jpg

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Wurstuk: Wurstuk uploaded a new version of File:Killifish life cycle.jpg

killifish life cycle

Killifish as an aging animal model

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Killifish as an aging animal model

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