Dmitry Dzhagarov: Created page with "Back in 1980, it was demonstrated that when cells are cultured, they release various proteins into the culture medium, which can affect the phenotype of other cells that enter this environment.Todaro, G. J., Fryling, C., & De Larco, J. E. (1980). Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proceedings of the National Academy of Sciences, 77(9), 5258-5262. PMID: 6254071 PMC35003..."

2024-04-07T19:36:59Z

Created page with "Back in 1980, it was demonstrated that when cells are cultured, they release various proteins into the culture medium, which can affect the phenotype of other cells that enter this environment.<ref>Todaro, G. J., Fryling, C., & De Larco, J. E. (1980). Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proceedings of the National Academy of Sciences, 77(9), 5258-5262. PMID: 6254071 PMC35003..."

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Back in 1980, it was demonstrated that when cells are cultured, they release various proteins into the culture medium, which can affect the phenotype of other cells that enter this environment.<ref>Todaro, G. J., Fryling, C., & De Larco, J. E. (1980). Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proceedings of the National Academy of Sciences, 77(9), 5258-5262. PMID: 6254071 PMC350037 DOI: 10.1073/pnas.77.9.5258</ref> In particular, teratocarcinoma cells are able to maintain a culture of pluripotent embryonic stem cell in an undifferentiated state, by supplying the culture medium with various factors.<ref>Martin, G. R. (1981). Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proceedings of the National Academy of Sciences, 78(12), 7634-7638. PMID: 6950406 PMC349323 DOI: 10.1073/pnas.78.12.7634</ref>
Factors provided by the '''niche''' are crucial ''in vivo''. For example, serial transplantation of somatic stem cells (SSCs) from male mice of different ages into young mice 3 months of age, showed that transplanted SSCs could be maintained '''far longer than their replicative lifespan for their age''' whereas SSCs from young mice could not be maintained when transplanted into old mice. These results points towards a deterioration of the SSC niche itself with aging rather than the loss of intrinsic factors in the SSC.<ref>Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL (2006). Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal. Stem Cells. 24 (6): 1505–1511. PMID 16456131 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501308/ PMC5501308] doi:10.1634/stemcells.2005-0580 </ref><ref>Farahzadi, R., Valipour, B., Montazersaheb, S., & Fathi, E. (2023). Targeting the stem cell niche micro-environment as therapeutic strategies in aging. Frontiers in Cell and Developmental Biology, 11, 1162136. PMID: 37274742 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235764/ PMC10235764] DOI: 10.3389/fcell.2023.1162136</ref>
Therefore, cells are often cultured on a feeder layer of young cells.<ref>Jang, Y. K., Jung, D. H., Jung, M. H., Kim, D. H., Yoo, K. H., Sung, K. W., ... & Yang, S. E. (2006). Mesenchymal stem cells feeder layer from human umbilical cord blood for ex vivo expanded growth and proliferation of hematopoietic progenitor cells. Annals of hematology, 85, 212-225. PMID: 16391912 DOI: 10.1007/s00277-005-0047-3</ref>

<ref>Fennel, Z. J., Bourrant, P. E., Kurian, A. S., Petrocelli, J. J., de Hart, N. M., Yee, E. M., ... & Drummond, M. J. (2024). [https://onlinelibrary.wiley.com/doi/10.1111/acel.14144 Stem cell secretome treatment improves whole‐body metabolism, reduces adiposity, and promotes skeletal muscle function in aged mice]. Aging Cell, e14144. PMID: 38500398 [https://doi.org/10.1111/acel.14144 DOI: 10.1111/acel.14144]</ref>

== References ==
<references />
[[Category:Main list]]
[[Category:Aging pathways and hallmarks]]
[[Category:Lifespan interventions]]
[[Category:Drafts]]

Secretome treatment - Revision history