Secretome treatment
Back in 1980, it was demonstrated that when cells are cultured, they release various proteins into the culture medium, which can affect the phenotype of other cells that enter this environment.[1] In particular, teratocarcinoma cells are able to maintain a culture of pluripotent embryonic stem cell in an undifferentiated state, by supplying the culture medium with various factors.[2] Factors provided by the niche are crucial in vivo. For example, serial transplantation of somatic stem cells (SSCs) from male mice of different ages into young mice 3 months of age, showed that transplanted SSCs could be maintained far longer than their replicative lifespan for their age whereas SSCs from young mice could not be maintained when transplanted into old mice. These results points towards a deterioration of the SSC niche itself with aging rather than the loss of intrinsic factors in the SSC.[3][4] Therefore, cells are often cultured on a feeder layer of young cells.[5]
References
- ↑ Todaro, G. J., Fryling, C., & De Larco, J. E. (1980). Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proceedings of the National Academy of Sciences, 77(9), 5258-5262. PMID: 6254071 PMC350037 DOI: 10.1073/pnas.77.9.5258
- ↑ Martin, G. R. (1981). Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proceedings of the National Academy of Sciences, 78(12), 7634-7638. PMID: 6950406 PMC349323 DOI: 10.1073/pnas.78.12.7634
- ↑ Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL (2006). Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal. Stem Cells. 24 (6): 1505–1511. PMID 16456131 PMC5501308 doi:10.1634/stemcells.2005-0580
- ↑ Farahzadi, R., Valipour, B., Montazersaheb, S., & Fathi, E. (2023). Targeting the stem cell niche micro-environment as therapeutic strategies in aging. Frontiers in Cell and Developmental Biology, 11, 1162136. PMID: 37274742 PMC10235764 DOI: 10.3389/fcell.2023.1162136
- ↑ Jang, Y. K., Jung, D. H., Jung, M. H., Kim, D. H., Yoo, K. H., Sung, K. W., ... & Yang, S. E. (2006). Mesenchymal stem cells feeder layer from human umbilical cord blood for ex vivo expanded growth and proliferation of hematopoietic progenitor cells. Annals of hematology, 85, 212-225. PMID: 16391912 DOI: 10.1007/s00277-005-0047-3
- ↑ Fennel, Z. J., Bourrant, P. E., Kurian, A. S., Petrocelli, J. J., de Hart, N. M., Yee, E. M., ... & Drummond, M. J. (2024). Stem cell secretome treatment improves whole‐body metabolism, reduces adiposity, and promotes skeletal muscle function in aged mice. Aging Cell, e14144. PMID: 38500398 DOI: 10.1111/acel.14144
