Lipofuscin

From Longevity Wiki

Lipofuscin is a yellow-brown autofluorescent pigment also known as "aging pigment" due to its age-related progressive accumulation. It is a waste product consisting of insoluble granules made of lipids and proteins that accumulate in the lysosomes of cells. Over time, the lysosome becomes clogged and is not able to continue working properly.[1][2][3] In motor neurons of centenarians, up to 75% of cell volume can be occupied by lipofuscin.[4]

Lipofuscin spots on the upper surface of the hands.

Lipofuscin is proposed as a senescent marker in long-lived, non-dividing cells of different tissues across species. However, it is not 100% specific to senescent cells, as it can accumulate in conditions such as age-related macular degeneration (AMD).[5] Lipofuscin accumulation in the lysosomes cause dysregulation and reduction of its autophagic capacity, generating ROS (reactive oxygen species), elevating lysosomal pH and leading to lysosome leakage.[6] Lipofuscin consists of a non-degradable intralysosomal substance, which forms mainly due to iron-catalyzed oxidation/polymerization of misfolded proteins (~30–70%) and lipid (~20–50%) residues together with metals such as iron, copper, zinc, manganese, and calcium, in a concentration up to 2%.[7][8][9][10][11]

Accumulation of lipofuscin or "aging pigment" is part of normal aging, and should be distinguished from accumulation of ceroid - autofluorescent storage material associated with disease and usually produced under various pathological conditions not necessarily related to aging.[12] Ceroid has been suggested to jeopardize cell performance and viability by inducing membrane fragility, mitochondrial dysfunction, DNA damage, and oxidative stress-induced apoptosis.[13]

Detection of lipofuscin

During the process of aging, lipofuscin accumulates in a nearly linear way in postmitotic senescencent cells (cardiomyocytes, retinal epithelial pigment cells, hepatocytes, neurons and keratinocytes),[14] and has been proposed as a detectable "marker" to estimate aging. This approach is particularly used to determine the age of crabs and other crustaceans either by labor histological fluorescent-microscopy examinations[15] or simply by extractable lipofuscin solvent fluorescence measurements.[16]

Detection of lipofuscin content can be used as a biomarker of old lysosome accumulation, either by its typical autofluorescence properties and fluorescence-based methods, or by selective staining with Sudan black B, which stained lipofuscin granules, allowing for detection in cells, tissues and body fluids.[17][18][19][20]

Relation to aging diseases

Because lipofuscin is a covalently cross-linked aggregate, it cannot be removed from the cytosol by the ubiquitin-proteasome system.[21][22] Furthermore, lipofuscin could belong to Advanced glycation end products (AGEs) deposits.[23]

Isolated lipofuscin aggregates, as shown in vitro, were readily incorporated by fibroblasts and caused cell death at low concentrations (LC50 = 5.0 µg/mL) via a pyroptosis-like pathway. Lipofuscin boosted mitochondrial ROS production and caused lysosomal dysfunction by lysosomal membrane permeabilization leading to reduced lysosome quantity and impaired cathepsin D activity.[24]

Lipofuscin granules accumulation can lead to pathology and accelerate the aging process.[25] The rate of lipofuscin formation has been shown to be negatively correlated with the life expectancy of postmitotic cells, i.e., the higher the rate, the shorter the lifespan of the cell due to decrease of cellular adaptability.[26] Therefore, progressive deposition of lipofuscin might promote the development of age-related pathologies, including macular degeneration, heart failure, and neuro-degenerative diseases.

Dry AMD

One of the diseases associated with the accumulation of lipofuscin is dry age-related macular degeneration (dry AMD) – a disease often diagnosed in people over 70 years of age and a leading cause of rapid vision loss. Dry AMD is a slow-progressing disease in which yellow drusen containing lipofuscin are deposited between the retinal pigment epithelial (RPE) cell layer and Bruch’s membrane.[27] A phototoxic components of lipofuscin such as A2E (Bis-retinoid N-retinyl-N-retinylidene ethanolamine) that induces inflammation and apoptosis in RPE cells,[28] are accumulated with age and mediate damage under blue light exposure.[29][30] It has been reported that iron levels increase in RPE during ageing and this intracellular iron can interact with bisretinoid lipofuscin in RPE to promote cell damage.[31] Therefore, to alleviate the deteriorating effects of lipofuscin on age-related macular degeneration, iron chelation, either independently or in combination with bisretinoid inhibitors could potentially serve as AMD treatments.[32] To protect human RPE cells from oxidative damage, caused by reactive oxygen species generated by the photo-excited lipofuscin, also is able L‐Citrulline, a naturally occurring amino acid with known antioxidant properties[33] and the main active component of Spirulina maxima P-phycocyanin - pigment with anti-inflammatory and antioxidant activities.[34]

The drug Lysoclear is an enzyme developed to enter RPE cells and break down lipofuscin deposits in the lysosomes, a therapeutic approach that proposes to reverse dry age-related macular degeneration and Stargardt's macular degeneration.[35] Phase 1 clinical trials started in 2018.

Zinc deficiency

Zinc-deficient animals showed a greater number of lipofuscin granules.[36] The relationship between zinc deficiency and enhanced lipofuscin accumulation suggest that zinc deficiency may result in the accumulation of substrates for autophagy whereas low zinc does not stimulate autophagy.[37] Autophagy is also inhibited when A2E-treated RPE cells are exposed to blue light.[30] Currently, the only intervention available for the treatment of dry AMD is Age-Related Eye Disease Supplement (AREDS), an oral supplement containing vitamin C, vitamin E, lutein/zeaxanthin, and zinc. It was shown that AREDS can reduce the risk of advanced AMD by about 25% over a 5-year period in patients with intermediate AMD.[38]

Lipofuscin accumulation in aging heart

Lipofuscin granules are found abundantly in myocardial cells.[39][40][41] The myocardial tissues of mice have the ability to eliminate the lipofuscin produced in the cardiomyocytes into the myocardial blood circulation. It is mainly carried out of cardiomyocytes into the myocardial interstitium in the form of small lipofuscin granules, using capsule-like protrusions that are formed on the sarcolemma.[42]

Role of lipofuscin in age-related neurodegeneration

Lipofuscin aggregation represents a risk factor for neurodegeneration.[43]

Progranulin neurons that normally have high levels of progranulin expression are more susceptible to age-related pathology, such as neuronal lipofuscinosis, in GRN−/− mice.[44]

Lipofuscin granules (brown) accumulation in aged (60y) human epidermis according to Rübe et al., & Scherthan (2021).[45]

Lipofuscin-accumulating in skin cells

Lipofuscin is an endogenous photosensitizer that efficiently absorbs ultraviolet radiation and visible light, forming electronic excited states that transfer energy to surrounding molecules. It is assumed, that photosensitized lipofuscin is cytotoxic because of its ability to incorporate redox-active transition metals (Fe+2), resulting in a redox-active surface, able to catalyze the Fenton reaction. Reactive oxygen and nitrogen species (ROS/RNS) generated by photosensitization of lipofuscin leads to DNA damage and strand breaks.[46][47] It was observed that application of vitamin E may reduce the level of lipofuscin in skin biopsies as well as lighten the skin (but not in very old ones).[48][14] Light-induced skin damage can be protected by regulating the ROS-ER stress-autophagy-apoptosis axis with hydrogen sulfide (H2S).[49]

Inhibitors of lipofuscin accumulation

It was suggested that formation of A2E and other toxic lipofuscin bisretinoids, such as A2-DHP-PE (A2-dihydropyridinephosphatidyl-ethanolamine) and atRALdi-PE (all-trans-retinal dimer phosphatidylethanolamine), occurs in the retina in a non-enzymatic manner and can be considered a by-product of a properly functioning visual cycle.[50] The formation of A2E was completely inhibited in total darkness, so, humans with retinal or macular degeneration may slow progression of their disease by limiting exposure to light.[51]

Meclofenoxate

Meclofenoxate is a cholinergic nootropic also known as centrophenoxine. It is an ester of dimethylethanolamine (DMAE) and 4-chlorophenoxyacetic acid (pCPA). Meclofenoxate, as well as DMAE, have been found to increase the dissolution and removal of lipofuscin[52][53][54] leading to lifespan extension of mice.[55][56] As a result of meclophenoxate treatment, a gradual decrease in the myocardial volume occupied by the pigment was noted. After 4-6 weeks of treatment, the pigment bodies were found lodged into the capillary endothelium and the lumen, facilitating the removal of the pigment via blood stream.[57][58]

Remofuscin

Remofuscin, a small molecule belonging to the tetrahydropyridoether class of compounds is able to remove lipofuscin from the RPE by accumulation specifically in RPE pigments and thus stimulation of the exocytosis.[59] Remofuscin formerly known as Soraprazan a potent and reversible selective inhibitor of gastric H,K-ATPase may be a promising drug candidate to manage neurodegenerative diseases related to lipofuscin accumulation.[60] Remofuscin reverses lipofuscin accumulation in aged primary human RPE cells and is non-cytotoxic in aged SD mouse RPE cells in vitro.[61] Mechanism causing lipofuscinolysis may involve the reactive oxygen species generated via the presence of remofuscin. Remofuscin binds to lipofuscin and is a superoxide generator when illuminated with light. Superoxide might help to degrade the polymeric lipofuscin into smaller units which then are transported out of the lysosomes by exocytosis.[61][62] Remofuscin reduces existing levels of lipofuscin in the RPE instead of merely slowing down accumulation of further toxic Vitamin A aggregates.[63][62]

Aging biomarkers were improved in remofuscin-treated Caenorhabditis elegans worms, resulting in a significant (p <0.05) increase in their lifespan.[62] The expression levels of genes related to lysosomes, a nuclear hormone receptor, fatty acid beta-oxidation, and xenobiotic detoxification were increased in remofuscin-treated worms. Moreover, remofuscin failed to extend the lives of C. elegans with loss-of-function mutations of genes related to lysosomes and xenobiotic detoxification, suggesting that these genes are associated with lifespan extension in remofuscin-treated C. elegans.[62]

Curcumin

Intestinal lipofuscin levels were reduced by 39.5% and 47.5%, respectively, in curcumin-treated adult C. elegans day-4 and -8 days of adulthood nematodes, compared to untreated controls.[64] This ability of curcumin is apparently not related to its action as a senolytics, since the potent senolytic fisetin, although it removed old cells, did not affect lipofuscin levels.[65]

NMDA receptor antagonists

N-methyl-D-aspartate (NMDA) receptors signaling is a novel mechanism for scavenging N-retinylidene-N-retinylethanolamine (A2E), a component of ocular lipofuscin, in human RPE cells. NMDA receptor antagonists, such as Ro 25-6981, CP-101,606 and AZD6765, degrade lipofuscin via autophagy in human RPE cells.[66] Ro 25-6981 has not yet been approved for clinical use. Among the clinically approved NMDA antagonists, memantine and ifenprodil have been proposed as drug repositioning to remove N-retinylidene-N-retinylethanolamine (A2E), an intracellular lipofuscin component.[67]

ATM inhibition

The increase in lipid peroxidation during oxidative stress increases the content of intra-lysosomal lipofuscins in fibroblasts during senescence.[68] Senescence amelioration in normal aging cells is mediated by the recovered mitochondrial function upon inhibition of a key mediator of DNA damage signaling and repair - Ataxia telangiectasia mutated (ATM).[69][70][71]

ATM inhibitors KU-60019, CP-466722 or antioxidant N-acetyl-cysteine (NAC) significantly reduced lipofuscin accumulation.[72]

See also

  • Renteln, M. (2024). Toward systemic lipofuscin removal. Rejuvenation Research, (ja). https://doi.org/10.1089/rej.2024.0034
  • Ilie, O. D., Ciobica, A., Riga, S., Dhunna, N., McKenna, J., Mavroudis, I., ... & Riga, D. (2020). Mini-review on lipofuscin and aging: focusing on the molecular interface, the biological recycling mechanism, oxidative stress, and the gut-brain axis functionality. Medicina, 56(11), 626. PMID: 33228124 PMCID: PMC7699382 DOI: 10.3390/medicina56110626
  • Nasiri, L., Vaez-Mahdavi, M. R., Hassanpour, H., Ghazanfari, T., Ardestani, S. K., Askari, N., ... & Rahimlou, B. (2023). Increased serum lipofuscin associated with leukocyte telomere shortening in veterans: a possible role for sulfur mustard exposure in delayed-onset accelerated cellular senescence. International Immunopharmacology, 114, 109549. https://doi.org/10.1016/j.intimp.2022.109549 Chronic oxidative stress and continuous inflammatory stimulation in veterans, due to mustard gas poisoning once in 1987, led to cells senescence with increased lipofuscin, and telomere shortening.
  • Nociari, M. M., Lehmann, G. L., Perez Bay, A. E., Radu, R. A., Jiang, Z., Goicochea, S., ... & Rodriguez-Boulan, E. (2014). Beta cyclodextrins bind, stabilize, and remove lipofuscin bisretinoids from retinal pigment epithelium. Proceedings of the National Academy of Sciences, 111(14), E1402-E1408. PMID: 24706818 PMCID: PMC3986126 DOI: 10.1073/pnas.1400530111

References

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  2. ↑ Reichel, W. (1968). Lipofuscin pigment accumulation and distribution in five rat organs as a function of age. Journal of gerontology, 23(2), 145-153. DOI: 10.1093/geronj/23.2.145
  3. ↑ Mann, D. M. A., Yates, P. O., & Stamp, J. E. (1978). The relationship between lipofuscin pigment and ageing in the human nervous system. Journal of the Neurological Sciences, 37(1-2), 83-93. DOI: 10.1016/0022-510x(78)90229-0
  4. ↑ Yin, D. (1996). Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores. Free Radical Biology and Medicine, 21(6), 871-888. PMID: 8902532 DOI: 10.1016/0891-5849(96)00175-x
  5. ↑ Georgakopoulou EA, Tsimaratou K, Evangelou K, Fernandez Marcos PJ, Zoumpourlis V, Trougakos IP, Kletsas D, Bartek J, Serrano M, Gorgoulis VG. Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues. Aging (Albany NY). 2013 Jan;5(1):37-50. doi: 10.18632/aging.100527.
  6. ↑ Dutta, R. K., Lee, J. N., Maharjan, Y., Park, C., Choe, S. K., Ho, Y. S., ... & Park, R. (2022). Catalase-deficient mice induce aging faster through lysosomal dysfunction. Cell Communication and Signaling, 20(1), 1-22. PMID:36474295 PMC9724376 DOI: 10.1186/s12964-022-00969-2
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  45. ↑ Rübe, C. E., Bäumert, C., Schuler, N., Isermann, A., Schmal, Z., Glanemann, M., ... & Scherthan, H. (2021). Human skin aging is associated with increased expression of the histone variant H2A. J in the epidermis. npj Aging and Mechanisms of Disease, 7(1), 1-11 PMID:33795696 PMC8016850 DOI:10.1038/s41514-021-00060-z
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